Optical melting measurements of nucleic acid thermodynamics.
نویسندگان
چکیده
Optical melting experiments provide measurements of thermodynamic parameters for nucleic acids. These thermodynamic parameters are widely used in RNA structure prediction programs and DNA primer design software. This review briefly summarizes the theory and underlying assumptions of the method and provides practical details for instrument calibration, experimental design, and data interpretation.
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عنوان ژورنال:
- Methods in enzymology
دوره 468 شماره
صفحات -
تاریخ انتشار 2009